CD81 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag

Cat: MG50743-CF
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All CD81 Reagents
CRO Services
Product recommended

All CD81 Reagents
CD81 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag 基本信息
基因
种属
Mouse
NCBI 参考序列号
参考序列ORF长度
750 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Mouse CD81 antigen with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-FLAG
限制性酶切位点
KpnI + XbaI(6kb+0.75kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
CD81 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD81 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Validated Images
other Green fluorescent protein / GFP Gene Plasmid Map 5609
Mouse CD81/TAPA1 Gene Expression validated Image 16070
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
CD81 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Alternative Names
Tapa-1 cDNA ORF Clone, Mouse;Tapa1 cDNA ORF Clone, Mouse;Tspan28 cDNA ORF Clone, Mouse
CD81 Background Information

CD81, also known as TAPA-1, belongs to the transmembrane 4 superfamily, also known as the tetraspanin family. Members of this family mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.CD81 is a widely expressed cell-surface protein involved in an astonishing variety of biologic responses. It is related to adhesion, morphology, activation, proliferation, and differentiation of B, T, and other cells. On B cells CD81 is part of a complex with CD21, CD19, and Leu13. This complex reduces the threshold for B cell activation via the B cell receptor by bridging Ag specific recognition and CD21-mediated complement recognition.

Full Name
CD81 molecule
References
  • Petracca R. et al., 2000, J Virol. 74 (10): 4824-30.
  • Bartosch B. et al., 2003, The Journal of Biological Chemistry. 278 (43): 41624-30.
  • Clark KL. et al., 2001, Journal of Immunology. 167 (9): 5115-21.
  • CD81 cDNA ORF Clone, Rhesus, Related Products

    Product Name Catalog#(PDF)
    CD81 cDNA ORF Clone in Cloning Vector, Rhesus CG90201-G
    CD81 cDNA ORF Clone, Rhesus, untagged CG90201-UT
    CD81 cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90201-ACG
    CD81 Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90201-ACGLN
    CD81 cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90201-ACR
    CD81 cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90201-CF
    CD81 cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90201-NF
    CD81 cDNA ORF Clone, Rhesus, C-His tag CG90201-CH
    CD81 cDNA ORF Clone, Rhesus, N-His tag CG90201-NH
    CD81 cDNA ORF Clone, Rhesus, C-HA tag CG90201-CY
    CD81 cDNA ORF Clone, Rhesus, N-HA tag CG90201-NY
    CD81 cDNA ORF Clone, Rhesus, C-Myc tag CG90201-CM
    CD81 cDNA ORF Clone, Rhesus, N-Myc tag CG90201-NM

    CD81 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    CD81 cDNA ORF Clone in Cloning Vector, Rat RG80331-G
    CD81 cDNA ORF Clone, Rat, untagged RG80331-UT
    CD81 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80331-ACGLN
    CD81 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80331-ACG
    CD81 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80331-ACR
    CD81 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80331-CF
    CD81 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80331-NF
    CD81 cDNA ORF Clone, Rat, C-His tag RG80331-CH
    CD81 cDNA ORF Clone, Rat, N-His tag RG80331-NH
    CD81 cDNA ORF Clone, Rat, C-HA tag RG80331-CY
    CD81 cDNA ORF Clone, Rat, N-HA tag RG80331-NY
    CD81 cDNA ORF Clone, Rat, C-Myc tag RG80331-CM
    CD81 cDNA ORF Clone, Rat, N-Myc tag RG80331-NM
    CD81 qPCR Primer Pairs, Rat RP300301

    CD81 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    CD81 cDNA ORF Clone in Cloning Vector, Mouse MG50743-G
    CD81 cDNA ORF Clone, Mouse, untagged MG50743-UT
    CD81 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50743-ACG
    CD81 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50743-ACGLN
    CD81 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50743-ACR
    CD81 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50743-NF
    CD81 cDNA ORF Clone, Mouse, C-His tag MG50743-CH
    CD81 cDNA ORF Clone, Mouse, N-His tag MG50743-NH
    CD81 cDNA ORF Clone, Mouse, C-HA tag MG50743-CY
    CD81 cDNA ORF Clone, Mouse, N-HA tag MG50743-NY
    CD81 cDNA ORF Clone, Mouse, C-Myc tag MG50743-CM
    CD81 cDNA ORF Clone, Mouse, N-Myc tag MG50743-NM
    CD81 qPCR Primer Pairs, Mouse MP200718

    CD81 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    CD81 cDNA ORF Clone in Cloning Vector, Human HG14244-G
    CD81 cDNA ORF Clone, Human, untagged HG14244-UT
    CD81 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG14244-ACGLN
    CD81 cDNA ORF Clone, Human, C-GFPSpark® tag HG14244-ACG
    CD81 cDNA ORF Clone, Human, C-OFPSpark® tag HG14244-ACR
    CD81 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG14244-CF
    CD81 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG14244-NF
    CD81 cDNA ORF Clone, Human, C-His tag HG14244-CH
    CD81 cDNA ORF Clone, Human, N-His tag HG14244-NH
    CD81 cDNA ORF Clone, Human, C-HA tag HG14244-CY
    CD81 cDNA ORF Clone, Human, N-HA tag HG14244-NY
    CD81 cDNA ORF Clone, Human, C-Myc tag HG14244-CM
    CD81 cDNA ORF Clone, Human, N-Myc tag HG14244-NM
    CD81 qPCR Primer Pairs, Human HP102894
    info info
    添加购物车成功! 添加购物车失败!