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小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体)

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Mouse CKB cDNA Clone产品信息
NCBI注册码:NM_021273.4
参考序列ORF长度:1146bp
cDNA基因描述:Full length Clone DNA of Mus musculus creatine kinase, brain.
分子别称:Bck, Ck3, B-CK, Ck-3, Ckbb
分子种属:Mouse
载体:pCMV3-untagged
质粒:
限制性酶切位点:
Tag序列:
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicilin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体) on other vectors
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG51386-ACG1950
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG51386-ACR1950
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签MG51386-ANG1950
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签MG51386-ANR1950
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG51386-CF1770
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), C-His 标签MG51386-CH1770
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG51386-CM1770
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG51386-CY1770
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG51386-NF1770
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), N-His 标签MG51386-NH1770
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG51386-NM1770
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG51386-NY1770
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA(克隆载体)MG51386-U760
小鼠 CKB / Creatine kinase B type 基因ORF全长cDNA克隆(表达载体)MG51386-UT1770
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研究背景

CKB(Creatine kinase B type) contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It belongs to the ATP:guanido phosphotransferase family. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CKB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. CKB reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer in brain, smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone etc.

参考资料
  • Wienker TF, et al. (1985) A dominant mutation causing ectopic expression of the creatine kinase B gene maps on chromosome 14. Cytogenet Cell Genet. 40:776.
  • Mariman EC, et al. (1989) Complete nucleotide sequence of the human creatine kinase B gene. Nucleic Acids Res. 17(15):6385.
  • Bong S, et al. (2008) Structural studies of human brain-type creatine kinase complexed with the ADP–Mg2+–NO3−–creatine transition-state analogue complex. FEBS Letters. 582(28): 3959-65.
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