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小鼠 CKM / CK-MM 基因ORF全长cDNA克隆(表达载体)

  • other Green fluorescent protein / GFP Gene Plasmid Map 5613
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小鼠 CKM cDNA Clone产品信息
NCBI注册码:X03233.1
参考序列ORF长度:1146bp
cDNA基因描述:Full length Clone DNA of Mus musculus creatine kinase, muscle.
分子别称:MCK, Ckmm, M-CK
分子种属:Mouse
载体:pCMV3-untagged
质粒:pCMV3-mCKM
限制性酶切位点:KpnI + XbaI(6.1kb+1.15kb)
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 147C/T not causing the amino acid variation.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with CKM qPCR primers for gene expression analysis, MP201233 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Product nameProduct name
研究背景

CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type).

参考资料
  • Goldblatt H. 1969, Laboratory Investigation. 21 (2): 126-8.
  • Hekimsoy Zeliha. et al., 2005, Endocrine Research. 31 (3): 171-5.
  • Döring F. et al., 2011, Scand J Med Sci Sports. 21(6):841-5.
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    货号: MG51356-UT
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