CLEC4F cDNA ORF Clone, Mouse, untagged

Cat: MG50282-UT
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All CLEC4F Reagents
CRO Services
Product recommended

All CLEC4F Reagents
CLEC4F cDNA ORF Clone, Mouse, untagged 基本信息
基因
种属
Mouse
NCBI 参考序列号
参考序列ORF长度
1647 bp
描述
Full length Clone DNA of Mouse C-type lectin domain family 4, member f.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-untagged
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Ampicillin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CLEC4F cDNA ORF Clone, Mouse, untagged Alternative Names
Clecsf13 cDNA ORF Clone, Mouse;D18063 cDNA ORF Clone, Mouse;Kclr cDNA ORF Clone, Mouse;KUCR_MOUSE cDNA ORF Clone, Mouse
CLEC4F Background Information

CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F(mCLEC4F) is only expressed on F4/80+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in adult mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E10) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells.

Full Name
C-type lectin domain family 4, member F
References
  • Shie-Liang Edmond Hsieh, et al. (2009) CLEC4F, A Kupffer Cells Specific Marker, Is Critical for Presentation of Alfa-Galactoceromide to NKT Cells. The Journal of Immunology. 182:78.
  • Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5.
  • Bonaldo MF, et al. (1996) Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res. 1996 Sep;6(9):791-806.
  • CLEC4F cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    CLEC4F cDNA ORF Clone in Cloning Vector, Rat RG80215-G
    CLEC4F cDNA ORF Clone, Rat, untagged RG80215-UT
    CLEC4F cDNA ORF Clone, Rat, C-GFPSpark® tag RG80215-ACG
    CLEC4F Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80215-ACGLN
    CLEC4F cDNA ORF Clone, Rat, C-OFPSpark® tag RG80215-ACR
    CLEC4F cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80215-CF
    CLEC4F cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80215-NF
    CLEC4F cDNA ORF Clone, Rat, C-His tag RG80215-CH
    CLEC4F cDNA ORF Clone, Rat, N-His tag RG80215-NH
    CLEC4F cDNA ORF Clone, Rat, C-HA tag RG80215-CY
    CLEC4F cDNA ORF Clone, Rat, N-HA tag RG80215-NY
    CLEC4F cDNA ORF Clone, Rat, C-Myc tag RG80215-CM
    CLEC4F cDNA ORF Clone, Rat, N-Myc tag RG80215-NM
    CLEC4F qPCR Primer Pairs, Rat RP300207

    CLEC4F cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    CLEC4F cDNA ORF Clone in Cloning Vector, Mouse MG50282-M
    CLEC4F cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50282-ACG
    CLEC4F Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50282-ACGLN
    CLEC4F cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50282-ACR
    CLEC4F cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50282-CF
    CLEC4F cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50282-NF
    CLEC4F cDNA ORF Clone, Mouse, C-His tag MG50282-CH
    CLEC4F cDNA ORF Clone, Mouse, N-His tag MG50282-NH
    CLEC4F cDNA ORF Clone, Mouse, C-HA tag MG50282-CY
    CLEC4F cDNA ORF Clone, Mouse, N-HA tag MG50282-NY
    CLEC4F cDNA ORF Clone, Mouse, C-Myc tag MG50282-CM
    CLEC4F cDNA ORF Clone, Mouse, N-Myc tag MG50282-NM
    CLEC4F qPCR Primer Pairs, Mouse MP200306

    CLEC4F cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    CLEC4F cDNA ORF Clone, Human, untagged HG11901-UT
    CLEC4F Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11901-ACGLN
    CLEC4F qPCR Primer Pairs, Human HP104669
    info info
    添加购物车成功! 添加购物车失败!