|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50535-ACG|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50535-ACR|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG50535-ANG|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG50535-ANR|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50535-CF|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50535-CH|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50535-CM|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50535-CY|
|小鼠 CRABP2 基因ORF全长cDNA(克隆载体)||MG50535-M|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50535-NF|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50535-NH|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50535-NM|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50535-NY|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体)||MG50535-UT|
Mouse cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema.