( We provide with CRABP2 qPCR primers for gene expression analysis, MP200527 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50535-ACG|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50535-ACR|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG50535-ANG|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG50535-ANR|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50535-CF|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50535-CH|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50535-CM|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50535-CY|
|小鼠 CRABP2 基因ORF全长cDNA(克隆载体)||MG50535-M|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50535-NF|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50535-NH|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50535-NM|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50535-NY|
|小鼠 CRABP2 基因ORF全长cDNA克隆(表达载体)||MG50535-UT|
Cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema.