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小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签

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    小鼠 CSNK1A1 cDNA Clone产品信息
    NCBI注册码:NM_146087.2
    参考序列ORF长度:978bp
    cDNA基因描述:Full length Clone DNA of Mus musculus casein kinase 1, alpha 1 with C terminal HA tag.
    分子别称:CK1a, Csnk1a, MGC29354, MGC30571, 2610208K14Rik, 4632404G05Rik, 5430427P18Rik, Csnk1a1
    分子种属:Mouse
    载体:pCMV3-C-HA
    质粒:
    限制性酶切位点:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with CSNK1A1 qPCR primers for gene expression analysis, MP200811 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签 on other vectors
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG50840-ACG1950
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG50840-ACR1950
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签MG50840-ANG1950
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签MG50840-ANR1950
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG50840-CF1770
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), C-His 标签MG50840-CH1770
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG50840-CM1770
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG50840-CY1770
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA(克隆载体)MG50840-G760
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG50840-NF1770
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), N-His 标签MG50840-NH1770
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG50840-NM1770
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG50840-NY1770
    小鼠 CSNK1A1/Casein Kinase 1, alpha 1 基因ORF全长cDNA克隆(表达载体)MG50840-UT1770
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    研究背景

    Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at 'Ser-45'. CSNK1A1 may play a role in segregating chromosomes during mitosis.

    参考资料
  • Dubois, et al.,2002, FEBS Lett. (Netherlands) 517 (1-3): 167-71. 
  • Dubois, T et al.,2001, J. Biol. Chem.(United States) 276 (22): 18757-64.
  • Zhang, Yi et al., 2002, J. Biol. Chem. 277(20): 17706-12.
  • Wang,Z. et al., 2010, Med Sci Monit.16 (8):CR357-64.
  • Size / Price
    货号: MG50840-CY
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