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小鼠 Cystatin 7 / CST7 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签

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    小鼠 CST7 cDNA Clone产品信息
    NCBI注册码:NM_009977.2
    参考序列ORF长度:504bp
    cDNA基因描述:Full length Clone DNA of Mus musculus cystatin F (leukocystatin) with C terminal GFPSpark tag.
    分子别称:CMAP, MGC151424, MGC151426, Cst7
    分子种属:Mouse
    载体:pCMV3-C-GFPSpark
    质粒:
    限制性酶切位点:
    Tag序列:GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with CST7 qPCR primers for gene expression analysis, MP200262 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    GFPSpark Tag Info

    GFPSpark is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSpark is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity.

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    研究背景

    The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.

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    货号: MG50236-ACG
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    Datasheet & Documentation

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