( We provide with CXCL5 qPCR primers for gene expression analysis, MP200375 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50354-ACG|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50354-ACR|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50354-CF|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50354-CH|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50354-CM|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50354-CY|
|小鼠 CXCL5 基因ORF全长cDNA(克隆载体)||MG50354-M|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50354-M-H|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50354-NF|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50354-NH|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50354-NM|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50354-NY|
|小鼠 CXCL5 基因ORF全长cDNA克隆(表达载体)||MG50354-UT|
CXCL5 is a small cytokine belonging to the CXC chemokine family. CXC chemokines are particularly significant for leukocyte infiltration in inflammatory diseases. CXCL5 is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. It also can be detected in eosinophils, and can be inhibited with the type II interferon. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis. It stimulates the chemotaxis of neutrophils possesses angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2.