|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG52211-ACG|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG52211-ACR|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG52211-ANG|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG52211-ANR|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG52211-CF|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG52211-CH|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG52211-CM|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG52211-CY|
|小鼠 DARS 基因ORF全长cDNA(克隆载体)||MG52211-G|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG52211-NF|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG52211-NH|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG52211-NM|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG52211-NY|
|小鼠 DARS 基因ORF全长cDNA克隆(表达载体)||MG52211-UT|
Aspartate tRNA ligase 1, also known as DARS, is part of a multienzyme complex of aminoacyl-tRNA synthetases. It belongs to the class-II aminoacyl-tRNA synthetase family. DARS charges its cognate tRNA with aspartate during protein biosynthesis. DARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid(AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.