( We provide with DCN qPCR primers for gene expression analysis, MP201385 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG51501-ACG|
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG51501-ACR|
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG51501-CF|
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG51501-CH|
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG51501-CM|
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG51501-CY|
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG51501-NF|
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG51501-NH|
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG51501-NM|
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG51501-NY|
|小鼠 Decorin/DCN 基因ORF全长cDNA(克隆载体)||MG51501-U|
|小鼠 Decorin/DCN 基因ORF全长cDNA克隆(表达载体)||MG51501-UT|
Decorin is a ubiquitous small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a core protein and a covalently linked glycosaminoglycan chain which is either chondroitin sulfate (CS) or dermatan sulfate (DS). As a component of connective tissue, decorin interacts with several extracellular matrix components, such as type I collagen and fibronectin, and plays a role in matrix assembly. Decorin resides in the tumor microenvironment and affects the biology of various types of cancer by downregulating the activity of several receptors involved in cell growth and survival. Decorin binds to and modulates the signaling of the epidermal growth factor receptor and other members of the ErbB family of receptor tyrosine kinases. It exerts its antitumor activity by a dual mechanism: via inhibition of these key receptors through their physical downregulation coupled with attenuation of their signaling, and by binding to and sequestering TGFbeta. Decorin also modulates the insulin-like growth factor receptor and the low-density lipoprotein receptor-related protein 1, which indirectly affects the TGFbeta receptor pathway. Decorin plays significant roles in tissue development and assembly, as well as playing both direct and indirect signaling roles.