( We provide with ELANE qPCR primers for gene expression analysis, MP200071 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50204-ACG|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50204-ACR|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG50204-ANG|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG50204-ANR|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50204-CF|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50204-CH|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50204-CM|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50204-CY|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA(克隆载体)||MG50204-M|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50204-NF|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50204-NH|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50204-NM|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50204-NY|
|小鼠 ELA2/Neutrophil Elastase 基因ORF全长cDNA克隆(表达载体)||MG50204-UT|
Neutrophil elastase, also known as Elastase-2, Bone marrow serine protease, Medullasin, ELANE, and ELA2, is a serine proteinase in the same family as chymotrypsin and has broad substrate specificity. Secreted by neutrophils during inflammation, it destroys bacteria and host tissue. As with other serine proteinases, ELANE / ELA2 contains a charge relay system composed of the catalytic triad of histidine, aspartate, and serine residues that are dispersed throughout the primary sequence of the polypeptide but that are brought together in the three dimension conformation of the folded protein. ELANE / ELA2 is an important protease enzyme that when expressed aberrantly can cause emphysema or emphysematous changes. This involves breakdown of the lung structure and increased airspaces. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. ELANE / ELA2 hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. ELANE / ELA2 may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. ELANE / ELA2 degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Defects in ELANE are a cause of cyclic haematopoiesis (CH), also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Defects in ELANE are also the cause of autosomal dominant severe congenital neutropenia type 1 (SCN1) which is a heterogeneous disorder of hematopoiesis.