Endoglin / CD105 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag

Cat: MG50407-CF
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Endoglin / CD105 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag 基本信息
基因
种属
Mouse
NCBI 参考序列号
参考序列ORF长度
1959 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Mouse endoglin, transcript variant 3 with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI (6kb + 2kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
Endoglin / CD105 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Endoglin / CD105 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Validated Images
other Green fluorescent protein / GFP Gene Plasmid Map 5609
Mouse Endoglin/CD105  Gene Expression validated Image 14228
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
Endoglin / CD105 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Alternative Names
AI528660 cDNA ORF Clone, Mouse;AI662476 cDNA ORF Clone, Mouse;CD105 cDNA ORF Clone, Mouse;Endo cDNA ORF Clone, Mouse;S-endoglin cDNA ORF Clone, Mouse
Endoglin / CD105 Background Information

Endoglin, also known as CD105, is a type I  homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type I I receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.

Full Name
endoglin
References
  • Bellon T., et al.,(1993), Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23:2340-2345.
  • Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.
  • Gougos A., et al.,(1990), Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.J. Biol. Chem. 265:8361-8364.
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    Endoglin / CD105 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    Endoglin / CD105 cDNA ORF Clone in Cloning Vector, Mouse MG50407-M
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    Endoglin / CD105 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50407-ACG
    Endoglin / CD105 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50407-ACGLN
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    Endoglin / CD105 cDNA ORF Clone, Mouse, C-His tag MG50407-CH
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    Endoglin / CD105 cDNA ORF Clone, Mouse, N-HA tag MG50407-NY
    Endoglin / CD105 cDNA ORF Clone, Mouse, C-Myc tag MG50407-CM
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    Endoglin / CD105 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    Endoglin / CD105 cDNA ORF Clone in Cloning Vector, Human HG10149-M
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    Endoglin / CD105 cDNA ORF Clone, Human, N-His tag HG10149-NH
    Endoglin / CD105 cDNA ORF Clone, Human, C-HA tag HG10149-CY
    Endoglin / CD105 cDNA ORF Clone, Human, N-HA tag HG10149-NY
    Endoglin / CD105 cDNA ORF Clone, Human, C-Myc tag HG10149-CM
    Endoglin / CD105 cDNA ORF Clone, Human, N-Myc tag HG10149-NM
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