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小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA(克隆载体)

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Mouse ENO1 cDNA Clone产品信息
NCBI注册码:NM_023119.2
参考序列ORF长度:1305bp
cDNA基因描述:Full length Clone DNA of Mus musculus enolase 1, alpha non-neuron.
分子别称:Eno-1, MBP-1, AL022784, 0610008I15
分子种属:Mouse
载体:PGEM-T Vector
质粒:pGEM-mENO1
限制性酶切位点:
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutation: 34G>A(E12K)
测序引物:SP6 and T7 or M13-47 and RV-M
启动子:
Application:
抗生素(大肠杆菌):Ampicilin
抗生素(哺乳动物细胞):
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA(克隆载体) on other vectors
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG52810-ACG1950
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG52810-ACR1950
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签MG52810-ANG1950
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签MG52810-ANR1950
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG52810-CF1770
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), C-His 标签MG52810-CH1770
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG52810-CM1770
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG52810-CY1770
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA(克隆载体)MG52810-G760
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG52810-NF1770
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), N-His 标签MG52810-NH1770
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG52810-NM1770
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG52810-NY1770
小鼠 ENO1 / Enolase 1 / alpha-enolase 基因ORF全长cDNA克隆(表达载体)MG52810-UT1770
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参考资料
  • Capello M, et al. (2011) a-Enolase: a promising therapeutic and diagnostic tumor target. FEBS J. 278(7): 1064-74.
  • Kang HJ, et al. (2008) Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme. Acta Crystallogr D Biol Crystallogr. 64(Pt 6): 651-7.
  • Lopez-Alemany R, et al. (2005) Alpha-enolase plasminogen receptor in myogenesis. Front Biosci. 10: 30-6.
  • Ejeskdr K, et al. (2005) Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death. BMC Cancer. 5: 161.
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