Mouse JAM-A / Junctional Adhesion Molecule A HEK293 Overexpression Lysate

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Mouse JAM-A / Junctional Adhesion Molecule A HEK293 Overexpression Lysate: 产品信息

产品描述
This Mouse JAM-A / Junctional Adhesion Molecule A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of JAM-A / Junctional Adhesion Molecule A protein (Cat: 50463-M02H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Mouse
蛋白构建信息
A DNA sequence encoding the mouse F11R (NP_766235.1) extracellular domain (Met 1-Ala 242) was fused with the Fc region of human IgG1 at the C-terminus.
分子量
The secreted recombinant mouse F11R/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 457 amino acids and has a predicted molecular mass of 50.2 kDa. rmF11R/Fc monomer migrates as an is approximately 57 kDa band in SDS-PAGE under reducing conditions, with ~10% free Fc fragment.

Mouse JAM-A / Junctional Adhesion Molecule A HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse JAM-A / Junctional Adhesion Molecule A HEK293 Overexpression Lysate: 别称

Mouse 9130004G24 Overexpression Lysate; Mouse AA638916 Overexpression Lysate; Mouse ESTM33 Overexpression Lysate; Mouse JAM Overexpression Lysate; Mouse JAM-1 Overexpression Lysate; Mouse JAM-A Overexpression Lysate; Mouse Jcam Overexpression Lysate; Mouse Jcam1 Overexpression Lysate; Mouse Ly106 Overexpression Lysate

JAM-A / Junctional Adhesion Molecule A 背景信息

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.
全称
F11 receptor
参考文献
  • Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.
  • Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.
  • Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
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