( We provide with FGF10 qPCR primers for gene expression analysis, MP200226 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50196-ACG|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50196-ACR|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50196-CF|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50196-CH|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50196-CM|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50196-CY|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA(克隆载体)||MG50196-M|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50196-NF|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50196-NH|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50196-NM|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50196-NY|
|小鼠 FGF10 / KGF2 基因ORF全长cDNA克隆(表达载体)||MG50196-UT|
Fibroblast growth factor 10 (FGF10) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF10 is required for normal branching morphogenesis. It may play a role in wound healing. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.