( We provide with FLT3 qPCR primers for gene expression analysis, MP201069 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG51119-ACG|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG51119-ACR|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG51119-CF|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG51119-CH|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG51119-CM|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG51119-CY|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA(克隆载体)||MG51119-G|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG51119-NF|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG51119-NH|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG51119-NM|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG51119-NY|
|小鼠 FLT3/CD135/FLK-2 基因ORF全长cDNA克隆(表达载体)||MG51119-UT|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD135, also known as FLT-3, FLK-2, is a member of the CD system. CD135 is an important cell surface marker recognized by specific sets of antibodies to identify the types of hematopoietic (blood) progenitors in the bone marrow and it function to differentiate hematopoietic stem cells, which are CD135 negative, from multipotent progenitors, which are CD135 positive. CD135 is a receptor tyrosine kinase typeⅢ for the cytokine Flt3 ligand and activat signaling through second messengers by binding to Flt3. Signaling through CD135 is important for lymphocyte development. The encoding gene CD135 is a proto-oncogene to which mutations happened will lead to cancer such as leukemia.