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小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), C-HA 标签

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    小鼠 GALE cDNA Clone产品信息
    NCBI注册码:NM_178389.3
    参考序列ORF长度:1044bp
    cDNA基因描述:Full length Clone DNA of Mus musculus galactose-4-epimerase, UDP with C terminal HA tag.
    分子别称:AI323962, 2310002A12Rik
    分子种属:Mouse
    载体:pCMV3-C-HA
    质粒:
    限制性酶切位点:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with GALE qPCR primers for gene expression analysis, MP201643 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), C-HA 标签 on other vectors
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG51770-ACG1950
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG51770-ACR1950
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签MG51770-ANG1950
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签MG51770-ANR1950
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG51770-CF1770
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), C-His 标签MG51770-CH1770
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG51770-CM1770
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG51770-CY1770
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA(克隆载体)MG51770-G760
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG51770-NF1770
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), N-His 标签MG51770-NH1770
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG51770-NM1770
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG51770-NY1770
    小鼠 UDP galactose-4'-epimerase / GALE 基因ORF全长cDNA克隆(表达载体)MG51770-UT1770
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    Product nameProduct name
    研究背景

    UDP galactose-4'-epimerase, also known as GALE, enables the body to process a simple sugar called galactose, which is present in small amounts in many foods. Galactose is primarily part of a larger sugar called lactose, which is found in all dairy products and many baby formulas. UDP galactose-4'-epimerase catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. Defects in GALE causes epimerase-deficiency galactosemia (EDG), also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation.

    参考资料
  • Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
  • Lee KA. et al., 2011,. J Biol Chem. 286 (48): 41530-8.
  • McCorvie TJ. et al., 2012, Biochim Biophys Acta. 1822 (10): 1516-26.
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    货号: MG51770-CY
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