( We provide with GAPDH qPCR primers for gene expression analysis, MP200537 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG51221-ACG|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG51221-ACR|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG51221-ANG|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG51221-ANR|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG51221-CF|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG51221-CH|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG51221-CM|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG51221-CY|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG51221-NF|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG51221-NH|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG51221-NM|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG51221-NY|
|小鼠 GAPDH 基因ORF全长cDNA(克隆载体)||MG51221-U|
|小鼠 GAPDH 基因ORF全长cDNA克隆(表达载体)||MG51221-UT|
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.