快速订购

小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA(克隆载体)

    产品数据评论相关产品实验方法
    小鼠 GLRX cDNA Clone产品信息
    NCBI注册码:NM_053108.4
    参考序列ORF长度:324bp
    cDNA基因描述:Full length Clone DNA of Mus musculus glutaredoxin.
    分子别称:Grx1, Glrx1, TTase, C86710, D13Wsu156e
    分子种属:Mouse
    载体:PGEM-T Vector
    质粒:pGEM-mGLRX
    限制性酶切位点:
    Tag序列:
    序列信息:Identical with the Gene Bank Ref. ID sequence.
    测序引物:SP6 and T7 or M13-47 and RV-M
    启动子:
    Application:
    抗生素(大肠杆菌):Ampicillin
    抗生素(哺乳动物细胞):
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA(克隆载体) on other vectors
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG52947-ACG1950
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG52947-ACR1950
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签MG52947-ANG1950
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签MG52947-ANR1950
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG52947-CF1770
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), C-His 标签MG52947-CH1770
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG52947-CM1770
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG52947-CY1770
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA(克隆载体)MG52947-G760
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG52947-NF1770
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), N-His 标签MG52947-NH1770
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG52947-NM1770
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG52947-NY1770
    小鼠 glutaredoxin-1 / GRX1 / GLRX 基因ORF全长cDNA克隆(表达载体)MG52947-UT1770
     点击了解更多载体信息
    Product nameProduct name
    研究背景

    Glutaredoxin-1, also known as GRX1 and GLRX, belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. Glutaredoxin-1 functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. Glutaredoxin-1 exists in either a reduced or an oxidized form. Glutaredoxins function as electron carriers in the glutathione-dependent synthesis of deoxyribonucleotides by the enzymeribonucleotide reductase.

    参考资料
  • Holmgren A. et al., 1988, FEMS Microbiol Rev. 4 (4): 271-97.
  • Holmgren A. 1988, Biochem Soc Trans. 16 (2): 95-6.
  • Holmgren A. 1989, J Biol Chem. 264 (24): 13963-6.
  • Size / Price
    货号: MG52947-G
    目录价: 
    单价:      (You Save: )

    Datasheet & Documentation

    产品数据可能随批次变化,最新数据请详见随货说明书。
    请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”