Gremlin 2/GREM2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag

Cat: MG50123-CF

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Gremlin 2/GREM2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag 基本信息

基因

种属
Mouse
NCBI 参考序列号
参考序列ORF长度
507 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Mouse gremlin 2 homolog, cysteine knot superfamily (Xenopus laevis) with C terminal Flag tag.

质粒

启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI (6kb + 0.55kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

Gremlin 2/GREM2 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Gremlin 2/GREM2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Validated Images

Mouse Gremlin 2/GREM2 Gene Expression validated Image 2083
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
other Green fluorescent protein / GFP Gene Plasmid Map 5609

Gremlin 2/GREM2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Alternative Names

Gremlin2 cDNA ORF Clone, Mouse;Prdc cDNA ORF Clone, Mouse

Gremlin 2/GREM2 Background Information

Full Name
gremlin 2, DAN family BMP antagonist

Gremlin 2/GREM2 cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
Gremlin 2/GREM2 cDNA ORF Clone in Cloning Vector, Mouse MG50123-M
Gremlin 2/GREM2 cDNA ORF Clone, Mouse, untagged MG50123-UT
Gremlin 2/GREM2 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50123-ACGLN
Gremlin 2/GREM2 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50123-ACG
Gremlin 2/GREM2 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50123-ACR
Gremlin 2/GREM2 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50123-NF
Gremlin 2/GREM2 cDNA ORF Clone, Mouse, C-His tag MG50123-CH
Gremlin 2/GREM2 cDNA ORF Clone, Mouse, N-His tag MG50123-NH
Gremlin 2/GREM2 cDNA ORF Clone, Mouse, C-HA tag MG50123-CY
Gremlin 2/GREM2 cDNA ORF Clone, Mouse, N-HA tag MG50123-NY
Gremlin 2/GREM2 cDNA ORF Clone, Mouse, C-Myc tag MG50123-CM
Gremlin 2/GREM2 cDNA ORF Clone, Mouse, N-Myc tag MG50123-NM
Gremlin 2/GREM2 qPCR Primer Pairs, Mouse MP200153

Gremlin 2/GREM2 cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
Gremlin 2/GREM2 cDNA ORF Clone in Cloning Vector, Human HG10283-U
Gremlin 2/GREM2 cDNA ORF Clone, Human, untagged HG10283-UT
Gremlin 2/GREM2 cDNA ORF Clone, Human, C-GFPSpark® tag HG10283-ACG
Gremlin 2/GREM2 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10283-ACGLN
Gremlin 2/GREM2 cDNA ORF Clone, Human, C-OFPSpark® tag HG10283-ACR
Gremlin 2/GREM2 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10283-CF
Gremlin 2/GREM2 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10283-NF
Gremlin 2/GREM2 cDNA ORF Clone, Human, C-His tag HG10283-CH
Gremlin 2/GREM2 cDNA ORF Clone, Human, N-His tag HG10283-NH
Gremlin 2/GREM2 cDNA ORF Clone, Human, C-HA tag HG10283-CY
Gremlin 2/GREM2 cDNA ORF Clone, Human, N-HA tag HG10283-NY
Gremlin 2/GREM2 cDNA ORF Clone, Human, C-Myc tag HG10283-CM
Gremlin 2/GREM2 cDNA ORF Clone, Human, N-Myc tag HG10283-NM
Gremlin 2/GREM2 qPCR Primer Pairs, Human HP104857
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