|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), C端GFPSpark标签||MG52883-ACG|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), C端OFPSpark标签||MG52883-ACR|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), N端GFPSpark标签||MG52883-ANG|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), N端OFPSpark标签||MG52883-ANR|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), C端FLAG标签||MG52883-CF|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), C端His标签||MG52883-CH|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), C端Myc标签||MG52883-CM|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), C端HA标签||MG52883-CY|
|小鼠 GSTM2 基因全长ORF克隆||MG52883-G|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), N端FLAG标签||MG52883-NF|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), N端His标签||MG52883-NH|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), N端Myc标签||MG52883-NM|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), N端HA标签||MG52883-NY|
|小鼠 GSTM2 基因全长ORF克隆 (表达载体), 无标签||MG52883-UT|
Glutathione S-transferase Mu 2, also known as GST class-mu 2, GSTM2-2 and GSTM2, is a cytoplasm protein which belongs to the GST superfamily and Mu family. GSTM2 / GST4 contains one GST C-terminal domain and one GST N-terminal domain. The glutathione S-transferases (GSTs) are a multigene family of enzymes largely involved in the detoxification of chemicals. Eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. Butyrate, an important luminal component produced from fermentation of dietary fibers, is an efficient inducer of GSTs and especially of GSTM2. Butyrate may act chemoprotectively by increasing detoxification capabilities in the colon mucosa.