The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG52939-ACG|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG52939-ACR|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG52939-ANG|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG52939-ANR|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG52939-CF|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG52939-CH|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG52939-CM|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG52939-CY|
|小鼠 HAPLN1 基因ORF全长cDNA(克隆载体)||MG52939-G|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG52939-NF|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG52939-NH|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG52939-NM|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG52939-NY|
|小鼠 HAPLN1 基因ORF全长cDNA克隆(表达载体)||MG52939-UT|
Hyaluronan (HA) is a high MW glycosaminoglycan significantly involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. HAPLN1, also known as CRTL1 (Cartilage Link Protein 1, cLP ) and link protein, is a member of HA-binding protein (hyaladherins) family, and contains a common structural domain of about 100 amino acids that is termed a Link module with two α-helices and two antiparallel β-sheets. HAPLN1/CRTL1 stabilizes the interaction between hyaluronan (HA) and versican, two extracellular matrix components essential for cardiac development. Link module superfamily can be divided into three subgroups, and the HAPLN family are C domain-type proteins that have an extended structure with one N-terminal V-type Ig-like domain followed by two link modules. In cartilage, aggrecan forms - cLP stabilized aggregates with HA that provides the tissue with its load bearing properties. HAPLN1 is a component of follicular matrix, was shown to enhance cumulus-oocyte complex (COC) expansion in vitro. HAPLN1 may promote periovulatory granulosa cell survival, which would facilitate their differentiation into luteal cells.