HSC70 cDNA ORF Clone, Mouse, C-HA tag

Cat: MG51191-CY
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All HSC70 Reagents
CRO Services
Product recommended

All HSC70 Reagents
HSC70 cDNA ORF Clone, Mouse, C-HA tag 基本信息
基因
种属
Mouse
NCBI 参考序列号
参考序列ORF长度
1983 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 114G/A,414C/T,549C/T,1350C/A not causing the amino acid variation.
描述
Full length Clone DNA of Mouse heat shock protein 8 with C terminal HA tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-HA
限制性酶切位点
KpnI + XbaI(6kb+1.98kb)
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
HSC70 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

HSC70 cDNA ORF Clone, Mouse, C-HA tag Validated Images
other Green fluorescent protein / GFP Gene Plasmid Map 5612
HSC70 cDNA ORF Clone, Mouse, C-HA tag Alternative Names
2410008N15Rik cDNA ORF Clone, Mouse;Hsc70 cDNA ORF Clone, Mouse;Hsc71 cDNA ORF Clone, Mouse;Hsc73 cDNA ORF Clone, Mouse;Hsp73 cDNA ORF Clone, Mouse;Hspa10 cDNA ORF Clone, Mouse
HSC70 Background Information

HSPA8, also known as HSC70, is a member of the heat shock protein family due to homology with other heat shock proteins. The heat shock protein 70 family is comprised by both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 belongs to the heat-shock cognate subgroup. Members of the human heat-shock protein multigene family have several highly conserved proteins with structural and functional properties in common, but vary in the extent of their inducibility in response to metabolic stress. HSPA8 is constitutively expressed and performs functions related to normal cellular processes. This protein binds to nascent polypeptides to facilitate correct protein folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. HSPA8 acts as a repressor of transcriptional activation. It inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. It also is a ATPase that works with auxilin to remove clathrin coated vesicles. In neurons, synaptojanin is also an important protein involved in vesicle uncoating.

Full Name
heat shock 70kDa protein 8
References
  • Santacruz H, et al. (1997) Molecular characterization of a heat shock cognate cDNA of zebrafish, hsc70, and developmental expression of the corresponding transcripts. Dev Genet. 21:223-33.
  • Harhay G P, et al. (2005) Characterization of 954 bovine full-CDS cDNA sequences. BMC Genomics. 6: 166.
  • Goldfarb S, et al. (2006) Differential effects of Hsc70 and Hsp70 on the intracellular trafficking and functional expression of epithelial sodium channels. Proc Natl Acad Sci. 103(15):5817-22.
  • HSC70 cDNA ORF Clone, Rhesus, Related Products

    Product Name Catalog#(PDF)
    HSC70 cDNA ORF Clone in Cloning Vector, Rhesus CG90471-G
    HSC70 cDNA ORF Clone, Rhesus, untagged CG90471-UT
    HSC70 cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90471-ACG
    HSC70 Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90471-ACGLN
    HSC70 cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90471-ACR
    HSC70 cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90471-CF
    HSC70 cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90471-NF
    HSC70 cDNA ORF Clone, Rhesus, C-His tag CG90471-CH
    HSC70 cDNA ORF Clone, Rhesus, N-His tag CG90471-NH
    HSC70 cDNA ORF Clone, Rhesus, C-HA tag CG90471-CY
    HSC70 cDNA ORF Clone, Rhesus, N-HA tag CG90471-NY
    HSC70 cDNA ORF Clone, Rhesus, C-Myc tag CG90471-CM
    HSC70 cDNA ORF Clone, Rhesus, N-Myc tag CG90471-NM

    HSC70 cDNA ORF Clone, Canine, Related Products

    Product Name Catalog#(PDF)
    HSC70 cDNA ORF Clone in Cloning Vector, Canine DG70157-U

    HSC70 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    HSC70 cDNA ORF Clone in Cloning Vector, Rat RG80575-U
    HSC70 cDNA ORF Clone, Rat, untagged RG80575-UT
    HSC70 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80575-ACG
    HSC70 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80575-ACGLN
    HSC70 cDNA ORF Clone, Rat, N-GFPSpark® tag RG80575-ANG
    HSC70 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80575-ACR
    HSC70 cDNA ORF Clone, Rat, N-OFPSpark® tag RG80575-ANR
    HSC70 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80575-CF
    HSC70 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80575-NF
    HSC70 cDNA ORF Clone, Rat, C-His tag RG80575-CH
    HSC70 cDNA ORF Clone, Rat, N-His tag RG80575-NH
    HSC70 cDNA ORF Clone, Rat, C-HA tag RG80575-CY
    HSC70 cDNA ORF Clone, Rat, N-HA tag RG80575-NY
    HSC70 cDNA ORF Clone, Rat, C-Myc tag RG80575-CM
    HSC70 cDNA ORF Clone, Rat, N-Myc tag RG80575-NM
    HSC70 qPCR Primer Pairs, Rat RP300539

    HSC70 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    HSC70 cDNA ORF Clone in Cloning Vector, Mouse MG51191-G
    HSC70 cDNA ORF Clone, Mouse, untagged MG51191-UT
    HSC70 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51191-ACGLN
    HSC70 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51191-ACG
    HSC70 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51191-ANG
    HSC70 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51191-ACR
    HSC70 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51191-ANR
    HSC70 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51191-CF
    HSC70 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51191-NF
    HSC70 cDNA ORF Clone, Mouse, C-His tag MG51191-CH
    HSC70 cDNA ORF Clone, Mouse, N-His tag MG51191-NH
    HSC70 cDNA ORF Clone, Mouse, N-HA tag MG51191-NY
    HSC70 cDNA ORF Clone, Mouse, C-Myc tag MG51191-CM
    HSC70 cDNA ORF Clone, Mouse, N-Myc tag MG51191-NM
    HSC70 qPCR Primer Pairs, Mouse MP201075

    HSC70 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    HSC70 cDNA ORF Clone in Cloning Vector, Human HG11329-M
    HSC70 cDNA ORF Clone, Human, untagged HG11329-UT
    HSC70 cDNA ORF Clone, Human, C-GFPSpark® tag HG11329-ACG
    HSC70 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11329-ACGLN
    HSC70 cDNA ORF Clone, Human, N-GFPSpark® tag HG11329-ANG
    HSC70 cDNA ORF Clone, Human, C-OFPSpark® tag HG11329-ACR
    HSC70 cDNA ORF Clone, Human, N-OFPSpark® tag HG11329-ANR
    HSC70 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11329-CF
    HSC70 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11329-NF
    HSC70 cDNA ORF Clone, Human, C-His tag HG11329-CH
    HSC70 cDNA ORF Clone, Human, N-His tag HG11329-NH
    HSC70 cDNA ORF Clone, Human, C-HA tag HG11329-CY
    HSC70 cDNA ORF Clone, Human, N-HA tag HG11329-NY
    HSC70 cDNA ORF Clone, Human, C-Myc tag HG11329-CM
    HSC70 cDNA ORF Clone, Human, N-Myc tag HG11329-NM
    HSC70 qPCR Primer Pairs, Human HP101214
    info info
    添加购物车成功! 添加购物车失败!