输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!

快速订购

Text Size:AAA

小鼠 IL2 基因全长ORF克隆 (表达载体), N端FLAG标签

产品数据评论相关产品实验方法
Mouse IL2 cDNA Clone产品信息
NCBI注册码:NM_008366.2
参考序列ORF长度:510bp
cDNA基因描述:Full length Clone DNA of Mus musculus interleukin 2 with N terminal Flag tag.
分子别称:IL-2
分子种属:Mouse
载体:pCMV3-SP-N-FLAG
质粒:pCMV3-Flag-mIL2
限制性酶切位点:KpnI + XbaI (6kb + 0.54kb)
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Mouse IL2 基因质粒图
Mouse IL2 natural ORF mammalian expression plasmid, N-Flag tag
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Product nameProduct name
重组小鼠 CD122 / IL2RB / IL2 Receptor beta 蛋白 (His 标签)重组狗 IL3RA 蛋白 (His 标签)重组狗 IL2RB / IL2 Receptor beta 蛋白 (Fc 标签)重组大鼠 IL7 / interleukin 7 蛋白重组人 IL-15 / IL15 / Interleukin 15 蛋白 (His 标签)Mouse CD123 / IL3RA Protein (ECD, Fc Tag)重组人 IL3 / IL-3 蛋白 (His 标签)重组食蟹猴 / 恒河猴 IL21R / IL-21R 蛋白 (Fc 标签)重组人 IL3 / IL-3 蛋白重组小鼠 IL-21R / Il21R 蛋白 (ECD, His Tag)重组大鼠 IL9 / IL-9 蛋白 (His 标签)Rhesus CD122 / IL-2RB Protein (Fc Tag)重组人 IL-8 / CXCL8 Protein (aa 28-99)Rhesus CD122 / IL-2RB Protein (His Tag)重组小鼠 IL7 / Interleukin 7 蛋白重组人 IL-8 / CXCL8 蛋白 (aa 23-99, Fc 标签)重组人 IL-8 / CXCL8 蛋白 (aa 28-99, Fc 标签)重组人 IL-8 / CXCL8 蛋白 (aa 23-99)重组人 IL-8 / CXCL8 蛋白 (aa 28-99)重组人 IL2Ra / CD25 蛋白 (Fc 标签)重组人 IL2Ra / CD25 蛋白 (His 标签)重组人 IL13RA2 / IL13R 蛋白 (His & Fc 标签)重组人 IL13RA2 / CD213A2 蛋白 (His 标签)重组人 IL13 / ALRH 蛋白 (Fc 标签)重组人 IL13 / ALRH 蛋白重组人 IL5Ra / CD125 蛋白 (His 标签)重组人 IL4R / CD124 蛋白 (His 标签)重组人 CD131 / CSF2RB / IL3RB / IL5RB 蛋白 (His 标签)重组人 IL3RA / CD123 蛋白 (His & Fc 标签)重组人 IL3RA / CD123 蛋白 (His 标签)重组小鼠 CD123 / IL3RA 蛋白 (ECD, His 标签)重组人 IL2RG / CD132 蛋白 (Fc 标签)重组人 IL2RG / CD132 蛋白 (His 标签)重组人 Interleukin-21 / IL-21 蛋白重组人 CD122 / IL-2RB 蛋白 (Fc 标签)重组人 CD122 / IL-2RB 蛋白重组人 IL13RA1 蛋白 (His & Fc 标签)重组人 IL13RA1 蛋白 (His 标签)重组人 IL16 / Interleukin-16 蛋白 (His 标签)重组人 IL7RA / CD127 蛋白 (His & Fc 标签)重组食蟹猴 CD127 / IL-7RA 蛋白 (His 标签)重组人 IL7RA / CD127 蛋白 (His 标签)重组食蟹猴 IL13 / ALRH 蛋白 (His 标签)重组食蟹猴 IL13 / ALRH 蛋白重组人 Interleukin-32 / IL-32 蛋白 (isoform alpha, His 标签)重组人 IL-21R / Interleukin-21 Receptor 蛋白 (His 标签)重组人 IL7 / interleukin 7 蛋白重组人 IL-9 / Interleukin-9 蛋白 (His 标签)重组人 IL4 / Interleukin-4 蛋白重组人 IL-3 / Interleukin-3 蛋白 (His 标签)重组小鼠 IL-4R / CD124 蛋白 (ECD, His 标签)重组小鼠 IL4 / Interleukin-4 蛋白重组小鼠 IL-34 蛋白 (His 标签)重组小鼠 IL13RA2 / CD213A2 蛋白 (His & Fc 标签)重组小鼠 IL13RA2 / CD213A2 蛋白 (His 标签)重组小鼠 IL2RG 蛋白 (His & Fc 标签)重组小鼠 IL2RG / CD132 蛋白 (His 标签)重组小鼠 IL-13Ra1 蛋白 (His & Fc 标签)重组小鼠 IL13RA1 蛋白 (His 标签)重组小鼠 IL7RA / CD127 蛋白 (His 标签)重组小鼠 IL21 / Interleukin 21 蛋白重组小鼠 IL13 / ALRH 蛋白重组小鼠 IL2RA / CD25 蛋白 (His 标签)重组小鼠 Interleukin-2 / IL-2 蛋白重组小鼠 IL5 蛋白 (His 标签)重组小鼠 IL4 / Interleukin-4 蛋白 (Q136D, Y139D, His 标签)重组小鼠 IL5Ra / CD125 蛋白 (His 标签)重组狗 IL-8 / CXCL8 蛋白重组狗 Interleukin-2 / IL-2 蛋白 (147 Cys/Ser)重组狗 IL21 / Interleukin 21 蛋白重组狗 IL5 蛋白 (His 标签)重组狗 IL4 / Interleukin-4 蛋白重组狗 IL13RA2 / IL13R 蛋白 (His 标签)重组人 IL5 / Interleukin 5 蛋白重组大鼠 Interleukin-2 / IL-2 蛋白重组大鼠 IL9R / Interleukin 9 receptor 蛋白 (His 标签)重组大鼠 IL7R / IL7RA 蛋白 (Fc 标签)重组大鼠 IL7R / IL7RA 蛋白 (His 标签)重组大鼠 IL13RA1 蛋白 (Fc 标签)重组大鼠 IL-21R / Interleukin-21 Receptor 蛋白 (His 标签)重组大鼠 IL2RG / CD132 蛋白 (Fc 标签)重组大鼠 IL2RG / CD132 蛋白 (His 标签)重组大鼠 IL4R / Il4ra 蛋白 (Fc 标签)重组大鼠 IL4R / Il4ra 蛋白 (His 标签)重组大鼠 IL21 / Interleukin 21 蛋白 (His 标签)重组大鼠 IL13RA2 / IL13R 蛋白 (Fc 标签)重组大鼠 IL13RA2 / IL13R 蛋白 (His 标签)重组大鼠 IL3 / interleukin 3 蛋白 (His 标签)重组大鼠 CD131 / CSF2RB / IL3RB / IL5RB 蛋白 (Fc 标签)重组人 IL2 / Interleukin-2 蛋白 (L35M, L36S, C142A)重组食蟹猴 IL-21R / Interleukin-21 Receptor 蛋白 (His 标签)Human IL-15 / IL15 / Interleukin 15 Protein重组食蟹猴 IL2RA 蛋白 (Fc 标签)重组食蟹猴 IL2RA 蛋白 (His 标签)重组食蟹猴 IL2RA 蛋白重组食蟹猴 IL-8 / CXCL8 蛋白Human IL-8 / CXCL8 Protein (His Tag)重组小鼠 IL16 / Interleukin-16 蛋白 (His 标签)重组狗 IL13RA2 / IL13R 蛋白 (Fc 标签)
研究背景

Interleukin-2, also known as T-cell growth factor, TCGF, Aldesleukin and IL2, is a secreted protein which belongs to the IL-2 family. Interleukin-2 / IL-2 was the first interleukin molecule to be discovered. Interleukin-2 / IL-2 molecule was first purified to homogeneity by immunoaffinity chromatography by Kendall Smith and his team at Dartmouth Medical School. Interleukin-2 / IL-2 was also the first cytokine shown to mediate its effects via a specific IL-2 receptor, and it was also the first interleukin to be cloned and expressed from a complementary DNA (cDNA) library. Interleukin-2 / IL-2 was designated number 2 because Smith's data at the time indicated that IL-1, produced by macrophages, facilitates IL-2 production by T lymphocytes (T cells).
Interleukin-2 / IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2 / IL-2 is normally produced by the body during an immune response. When environmental substances (molecules or microbes) gain access to the body, these substances (termed antigens) are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2 / IL-2, and the expression of IL-2 receptors IL-2R. The IL-2 / IL-2R interaction then stimulates the growth, differentiation and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2 / IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The World Reference Standard for Interleukin-2 / IL-2 is produced by the National Institute of Biological Standards and Control in the UK. A recombinant form of Interleukin-2 / IL-2 for clinical use is manufactured by Chiron Corporation with the brand name Proleukin. It has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines. The use of Interleukin-2 / IL-2 in HIV therapy has been found to be ineffective.

参考资料
  • Smith KA, et al.,1980,  J. Exp. Med.151 (6): 1551-6. 
  • Smith KA, et al.,1980, Nature. 287 (5785): 853-5.
  • Taniguchi T, et al.,1983, Nature. 302 (5906): 305.
  • Cantrell DA, et al.,1984, Science. 224 (4655): 1312-6. 
  • Smith KA, et al.,1988, Science. 240 (4856): 1169-76.
  • Wang X. et al., 2005, Science 310:1159-63.
  • Stauber D.J. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 2788-93.
  • Size / Price
    货号: MG51061-NF
    目录价:   (Save )
    单价:      [How to order]
    库存In Stock运输说明
    产品数据可能随批次变化,最新数据请详见随货说明书。
    请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”