|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50792-ACG|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50792-ACR|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50792-CF|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50792-CH|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50792-CM|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50792-CY|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA(克隆载体)||MG50792-G|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50792-NF|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50792-NH|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50792-NM|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50792-NY|
|小鼠 CD122 / IL-2RB 基因ORF全长cDNA克隆(表达载体)||MG50792-UT|
Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.