输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!

快速订购

Text Size:AAA

小鼠 IL-33 基因ORF全长cDNA克隆(表达载体), N-His 标签

产品数据评论相关产品实验方法
Mouse IL33 cDNA Clone产品信息
NCBI注册码:NM_133775.1
参考序列ORF长度:801bp
cDNA基因描述:Full length Clone DNA of Mus musculus interleukin 33 with N terminal His tag.
分子别称:Il-33, Il1f11, NF-HEV, 9230117N10Rik
分子种属:Mouse
载体:pCMV3-N-His
质粒:pCMV3-His-mIL33
限制性酶切位点:HindIII + XbaI (6kb + 0.85kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Mouse IL33 基因质粒图
Mouse IL33 / NF-HEV natural ORF mammalian expression plasmid, N-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Product nameProduct name
研究背景

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial enules), is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.

参考资料
  • Iikura M, et al. (2007) IL-33 can promote survival, adhesion and cytokine production in human mast cells. Lab Invest. 87(10): 971-8.
  • Lamkanfi M, et al. (2009) IL-33 raises alarm. Immunity. 31(1): 5-7.
  • Size / Price
    货号: MG50118-NH
    目录价: 
    单价:      (You Save: )
    规格:
    数量:+-
    库存In Stock
    大包装查询加入购物车
    Contact Us
    产品图片
    • Mouse IL33 / NF-HEV natural ORF mammalian expression plasmid, N-His tag
      您曾浏览过的产品
        产品数据可能随批次变化,最新数据请详见随货说明书。
        请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”