|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG51071-ACG|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG51071-ACR|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG51071-CF|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG51071-CH|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG51071-CM|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG51071-CY|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA(克隆载体)||MG51071-G|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG51071-NF|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG51071-NH|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG51071-NM|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG51071-NY|
|小鼠 IL36B/IL1F8 基因ORF全长cDNA克隆(表达载体)||MG51071-UT|
Interleukin-1 family member 8(IL1F8) also known as IL36B, is a member of the interleukin 1(IL-1) cytokine family. IL1F8 may contains a 12-stranded beta-trefoil structure. Until recently, the IL-1 family of cytokines includ four members, with three having pro-inflammatory effects such as IL1F8 and the fourth member being an IL-1 receptor antagonist (IL-1Ra). IL-1 family members exert their effects through binding to receptors that belong to the IL-1 receptor (IL-1R) family. IL1F8 exerts proinflammatory effects in primary human joint cells. Joint and serum IL-1F8 protein levels did not correlate with inflammation, but they were high in some human serum samples tested, including samples from patients with rheumatoid arthritis. IL1F8 also activated NK-κB.