|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50213-ACG|
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50213-ACR|
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50213-CF|
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50213-CH|
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50213-CM|
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50213-CY|
|小鼠 IL-1F5 基因ORF全长cDNA(克隆载体)||MG50213-M|
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50213-NF|
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50213-NH|
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50213-NM|
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50213-NY|
|小鼠 IL-1F5 基因ORF全长cDNA克隆(表达载体)||MG50213-UT|
Interleukin-1 family member 5 (IL-1F5), also known as interleukin 36 receptor antagonist (IL36RA), is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). IL-1F5 could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), which is present in epithelial barriers and takes part in local inflammatory response. It has been proved that IL-1F5 induces IL-4 mRNA and protein expression in glia in vitro and enhances hippocampal expression of IL-4 following intracerebroventricular injection. The inhibitory effect of IL-1F5 on LPS-induced IL-1β is attenuated in cells from IL-4-defective mice. Experiment results suggest that IL-1F5 mediates anti-inflammatory effects through its ability to induce IL-4 production and that this is a consequence of its interaction with the orphan receptor, single Ig IL-1R-related molecule (SIGIRR)/TIR8, as the effects were not observed in SIGIRR−/− mice. In contrast to its effects in brain tissue, IL-1F5 did not attenuate LPS-induced changes, or up-regulated IL-4 in macrophages or dendritic cells, suggesting that the effect is confined to the brain.