( We provide with INSR qPCR primers for gene expression analysis, MP201019 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG51062-ACG|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG51062-ACR|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG51062-CF|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG51062-CH|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG51062-CM|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG51062-CY|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA(克隆载体)||MG51062-G|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG51062-NF|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG51062-NH|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG51062-NM|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG51062-NY|
|小鼠 Insulin Receptor/INSR/CD220 基因ORF全长cDNA克隆(表达载体)||MG51062-UT|
INSR (Insulin receptor), also known as CD220, is a transmembrane receptor that is activated by insulin. INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K). IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5). It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM).