|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50361-ACG|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50361-ACR|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG50361-ANG|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG50361-ANR|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50361-CF|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50361-CH|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50361-CM|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50361-CY|
|小鼠 ITK Kinase 基因ORF全长cDNA(克隆载体)||MG50361-M|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50361-NF|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50361-NH|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50361-NM|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50361-NY|
|小鼠 ITK Kinase 基因ORF全长cDNA克隆(表达载体)||MG50361-UT|
IL-2-inducible T cell kinase is a member of the protein kinase superfamily, Tyr protein kinase family and TEC subfamily. It contains 1 Btk-type zinc finger, 1 PH domain, 1 protein kinase domain, 1 SH2 domain and 1 SH3 domain. As an intracellular kinase which expressed in T-cells, IL-2-inducible T cell kinase contains both SH2 and SH3 domains which are often found in intracellular kinases. It is hought to play a role in T-cell proliferation and differentiation. It regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. IL-2-inducible T cell kinase also plays an essential role in regulation of the adaptive immune response. efects in IL-2-inducible T cell kinase are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1). LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.