( We provide with KIRREL qPCR primers for gene expression analysis, MP200223 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50193-ACG|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50193-ACR|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50193-CF|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50193-CH|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50193-CM|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50193-CY|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA(克隆载体)||MG50193-M|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50193-NF|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50193-NH|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50193-NM|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50193-NY|
|小鼠 KIRREL1/NEPH1 基因ORF全长cDNA克隆(表达载体)||MG50193-UT|
NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and in vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.