c-Kit cDNA ORF Clone, Mouse, untagged

1/1
Price:
Size:
Number:

c-Kit cDNA ORF Clone, Mouse, untagged: 产品信息

基因
种属
Mouse
NCBI 参考序列号
基因长度
2940 bp
序列特征
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1602T/C not causing the amino acid variation.
产品特征
Full length Clone DNA of Mouse kit oncogene.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI(6.1kb+2.94kb)
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Ampicillin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

c-Kit cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

c-Kit cDNA ORF Clone, Mouse, untagged: 验证图片

c-Kit cDNA ORF Clone, Mouse, untagged: 别称

Bs cDNA ORF Clone, Mouse; c-KIT cDNA ORF Clone, Mouse; CD117 cDNA ORF Clone, Mouse; Fdc cDNA ORF Clone, Mouse; Gsfsco1 cDNA ORF Clone, Mouse; Gsfsco5 cDNA ORF Clone, Mouse; Gsfsow3 cDNA ORF Clone, Mouse; SCO1 cDNA ORF Clone, Mouse; SCO5 cDNA ORF Clone, Mouse; SOW3 cDNA ORF Clone, Mouse; Ssm cDNA ORF Clone, Mouse; Tr-kit cDNA ORF Clone, Mouse; W cDNA ORF Clone, Mouse

c-Kit 背景信息

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.
全称
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
参考文献
  • Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
  • Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
  • Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
  • Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
  • McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后