|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50404-ACG|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50404-ACR|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50404-CF|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50404-CH|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50404-CM|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50404-CY|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA(克隆载体)||MG50404-M|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50404-NF|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50404-NH|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50404-NM|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50404-NY|
|小鼠 LGALS7 / Galectin-7 基因ORF全长cDNA克隆(表达载体)||MG50404-UT|
LGALS7, also known as Galectin-7, is a member of the galectins family. The galectins are a family of beta-galactoside-binding proteins. There are at least 14 identified members in this family. Galectins share similarities in the CRD (the carbohydrate recognition domain). They are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are implicated in modulating cell-cell and cell-matrix interactions. LGALS7 contains 1 galectin domain and is mainly expressed in stratified squamous epithelium. Galectin-7 could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. LGALS7 is a pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.