|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50423-ACG|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50423-ACR|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50423-CF|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50423-CH|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50423-CM|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50423-CY|
|小鼠 LIFR/CD118 基因ORF全长cDNA(克隆载体)||MG50423-M|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50423-M-H|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50423-NF|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50423-NH|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50423-NM|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50423-NY|
|小鼠 LIFR/CD118 基因ORF全长cDNA克隆(表达载体)||MG50423-UT|
LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (cardiotrophin like cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1 and cardiotrophin like cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.