( We provide with MAPK9 qPCR primers for gene expression analysis, MP200981 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG51022-ACG|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG51022-ACR|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG51022-ANG|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG51022-ANR|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG51022-CF|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG51022-CH|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG51022-CM|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG51022-CY|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA(克隆载体)||MG51022-G|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG51022-NF|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG51022-NH|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG51022-NM|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG51022-NY|
|小鼠 JNK2/MAPK9 基因ORF全长cDNA克隆(表达载体)||MG51022-UT|
Mitogen-activated protein kinase 9 (MAPK9), also well known as c-Jun N-terminal kinase (JNK2), is a member of MAP kinase subfamily belonging to the protein kinase superfamily. MAPK9 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, such as c-Jun and ATF2. The crystal structure of human JNK2 complexed with an indazole inhibitor by applying a high-throughput protein engineering and surface-site mutagenesis approach. A novel conformation of the activation loop is observed, which is not compatible with its phosphorylation by upstream kinases. This activation inhibitory conformation of JNK2 is stabilized by the MAP kinase insert that interacts with the activation loop in an induced-fit manner. It suggest that the MAP kinase insert of JNK2 plays a role in the regulation of JNK2 activation, possibly by interacting with intracellular binding partners. JNK2 deficiency leads to reduced c-Jun degradation, thereby augmenting c-Jun levels and cellular proliferation, and suggests that JNK2 is a negative regulator of cellular proliferation in multiple cell types. JNK2 prevents replicative stress by coordinating cell cycle progression and DNA damage repair mechanisms. JNK2 blocks the ubiquitination of tumor suppressor p53, and thus increases the stability of p53 in nonstressed cells. JNK2 negatively regulates antigen-specific CD8+ T cell expansion and effector function, and thus selectively blocking JNK2 in CD8+ T cells may potentially enhance anti-tumor immune response. Lack of JNK2 expression was associated with higher tumor aneuploidy and reduced DNA damage response. Additionally,the JNK2 protein could be a novel therapeutic target in dry eye disease, and may provide a novel target for prevention of vascular disease and atherosclerosis.