|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50794-ACG|
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50794-ACR|
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50794-CF|
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50794-CH|
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50794-CM|
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50794-CY|
|小鼠 CD146/MCAM 基因ORF全长cDNA(克隆载体)||MG50794-G|
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50794-NF|
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50794-NH|
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50794-NM|
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50794-NY|
|小鼠 CD146/MCAM 基因ORF全长cDNA克隆(表达载体)||MG50794-UT|
The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.