( We provide with MESDC2 qPCR primers for gene expression analysis, MP200229 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50200-ACG|
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50200-ACR|
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50200-CF|
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50200-CH|
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50200-CM|
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50200-CY|
|小鼠 MESDC2 基因ORF全长cDNA(克隆载体)||MG50200-M|
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50200-NF|
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50200-NH|
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50200-NM|
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50200-NY|
|小鼠 MESDC2 基因ORF全长cDNA克隆(表达载体)||MG50200-UT|
LDLR chaperone MESD, also known as Mesoderm development protein, Mesoderm development candidate 2, Renal carcinoma antigen NY-REN-61 and MESDC2, is a member of the MESD family. MESDC2 is a chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). The LDLR maturation activity resides in the N- and C-terminal unstructured regions. MESDC2 acts as a modulator of the Wnt pathway, since some LDLRs are coreceptors for the canonical Wnt pathway. MESDC2 is essential for specification of embryonic polarity and mesoderm induction.