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小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), C-Myc 标签

产品数据评论相关产品实验方法
Mouse MFF cDNA Clone产品信息
NCBI注册码:NM_029409.2
参考序列ORF长度:876bp
cDNA基因描述:Full length Clone DNA of Mus musculus mitochondrial fission factor with C terminal Myc tag.
分子别称:AI314724, 5230400G24Rik
分子种属:Mouse
载体:pCMV3-C-Myc
质粒:
限制性酶切位点:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), C-Myc 标签 on other vectors
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG51618-ACG1950
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG51618-ACR1950
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签MG51618-ANG1950
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签MG51618-ANR1950
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG51618-CF1770
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), C-His 标签MG51618-CH1770
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG51618-CM1770
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG51618-CY1770
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA(克隆载体)MG51618-G760
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG51618-NF1770
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), N-His 标签MG51618-NH1770
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG51618-NM1770
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG51618-NY1770
小鼠 MFF/mitochondrial fission factor 基因ORF全长cDNA克隆(表达载体)MG51618-UT1770
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货号: MG51618-CM
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库存2-3 weeks
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