( We provide with MMP9 qPCR primers for gene expression analysis, MP200552 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50560-ACG|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50560-ACR|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50560-CF|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50560-CH|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50560-CM|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50560-CY|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA(克隆载体)||MG50560-M|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50560-NF|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50560-NH|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50560-NM|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50560-NY|
|小鼠 MMP9/MMP-9/CLG4B 基因ORF全长cDNA克隆(表达载体)||MG50560-UT|
Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions, such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. MMP9, also known as 92-kDa gelatinase B/type IV collagenase, is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. It plays an important role in tissue remodelling in normal and pathological inflammatory processes. MMP-9 is a major secretion product of macrophages and a component of cytoplasmic granules of neutrophils, and is particularly important in the pathogenesis of inflammatory, infectious, and neoplastic diseases in many organs including the lung. This enzyme is also secreted by lymphocytes and stromal cells upon stimulation by inflammatory cytokines, or upon delivery of bi-directional activation signals following integrin-mediated cell-cell or cell-extracellular matrix (ECM) contacts. Since the integrity of the tissue architecture is closely dependent of the delicate balance between MMPs and their inhibitors, excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a consequence, regulation of gene transcription and tissue-specific expression of MMP-9 in normal and diseased states are being actively investigated to pave the way for new therapeutic targets. In addition, the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions clearly points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention.