|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG52768-ACG|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG52768-ACR|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG52768-ANG|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG52768-ANR|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG52768-CF|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG52768-CH|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG52768-CM|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG52768-CY|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA(克隆载体)||MG52768-G|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG52768-NF|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG52768-NH|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG52768-NM|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG52768-NY|
|小鼠 PARK7/DJ-1 基因ORF全长cDNA克隆(表达载体)||MG52768-UT|
Parkinson's disease locus DJ-1 (PARK7) is a differentially expressed transcript. DJ-1 plays a physiologic role in protection of erythroid cells from oxidant damage, a function unmasked in the context of oxidative stress. PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson's disease (PD). DJ-1/p53 interactions contribute to apoptosis resistance in clonal myeloid cells and may serve as a prognostic marker in patients with myelodysplastic syndromes (MDS). DJ-1 regulates redox signaling kinase pathways and acts as a transcriptional regulator of antioxidative gene batteries. Therefore, DJ-1 is an important redox-reactive signaling intermediate controlling oxidative stress after ischemia, upon neuroinflammation, and during age-related neurodegenerative processes. Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson's disease and acute damage such as stroke.