( We provide with PDCD1 qPCR primers for gene expression analysis, MP200154 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50124-ACG|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50124-ACR|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50124-CF|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50124-CH|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50124-CM|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50124-CY|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA(克隆载体)||MG50124-M|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50124-NF|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50124-NH|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50124-NM|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50124-NY|
|小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体)||MG50124-UT|
Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
Immune Checkpoint Blockade: Blocking Antibodies Immune Checkpoint Blockade: PD1 / PDCD1 / CD279 Blocking Antibodies
Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins PD1 / PDCD1 / CD279 Immune Checkpoint Proteins
PD1 / PDCD1 / CD279 Immune Checkpoint PD1 / PDCD1 / CD279 Immune Checkpoint Antibodies
Immune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets