NCBI注册码: NM_008798.1 参考序列ORF长度: 906 bp cDNA基因描述: Full length Clone DNA of Mus musculus programmed cell death 1 with N terminal His tag. 分子别称: PD-1, Pdc1, Ly101 分子种属: Mouse 载体: pCMV3-SP-N-His 质粒: pCMV3-His-mPDCD1 限制性酶切位点: HindIII + NotI(6kb+0.91kb) Tag序列: His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC 序列信息: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 771G/A not causing the amino acid variation. 测序引物: T7(TAATACGACTCACTATAGGG)
BGH(TAGAAGGCACAGTCGAGG) ( We provide with PDCD1 qPCR primers for gene expression analysis, MP200154 ) 启动子: Enhanced CMV mammalian cell promoter Application: Stable or Transient mammalian expression 抗生素（大肠杆菌）: Kanamycin 抗生素（哺乳动物细胞）: Hygromycin 运输方式: Each tube contains lyophilized plasmid. 储存方法: The lyophilized plasmid can be stored at room temperature for three months.
Mouse PD1 / PDCD1 natural ORF mammalian expression plasmid, N-His tag
Immunochemical staining of human CCNF in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Mammalian Expression Vector
Constiutive, Stable / Transient
Selection In Mammalian Cells
pCMV3-SP-N-His (suitable for secretary and membane protein expession)
Schematic of pCMV3-SP-N-His (suitable for secretary and membane protein expession) Multiple Cloning Sites
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-His 标签 on other vectors
小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签 MG50124-ACG ￥ 1950 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签 MG50124-ACR ￥ 1950 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-Flag 标签 MG50124-CF ￥ 1770 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-His 标签 MG50124-CH ￥ 1770 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-Myc 标签 MG50124-CM ￥ 1770 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-HA 标签 MG50124-CY ￥ 1770 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA(克隆载体) MG50124-M ￥ 760 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-Flag 标签 MG50124-NF ￥ 1770 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-His 标签 MG50124-NH ￥ 1770 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-Myc 标签 MG50124-NM ￥ 1770 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-HA 标签 MG50124-NY ￥ 1770 小鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体) MG50124-UT ￥ 1770
Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
Immune Checkpoint Immune Checkpoint Blockade: Blocking Antibodies Immune Checkpoint Blockade: PD1 / PDCD1 / CD279 Blocking Antibodies Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins PD1 / PDCD1 / CD279 Immune Checkpoint Proteins PD1 / PDCD1 / CD279 Immune Checkpoint PD1 / PDCD1 / CD279 Immune Checkpoint Antibodies Immune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets
Immunotherapy Cancer Immunotherapy Targeted Therapy James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7. Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24. del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28. Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25. Featured Reagent Products