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小鼠 PGF 基因ORF全长cDNA克隆(表达载体), N-His 标签

产品数据评论相关产品实验方法
Mouse PGF cDNA Clone产品信息
NCBI注册码:NM_008827.2
参考序列ORF长度:477bp
cDNA基因描述:Full length Clone DNA of Mus musculus placental growth factor with N terminal His tag.
分子别称:PIGF, Plgf, AI854365, Pgf
分子种属:Mouse
载体:pCMV3-SP-N-His
质粒:
限制性酶切位点:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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参考资料
  • Nagy JA, et al. (2003) VEGF-A(164/165) and PlGF: roles in angiogenesis and arteriogenesis. Trends Cardiovasc Med. 13(5): 169-75.
  • Chaballe L, et al. (2011) Placental growth factor: a tissue modelling factor with therapeutic potentials in neurology? Acta Neurol Belg. 111(1): 10-7.
  • Odorisio T, et al. (2006) The placenta growth factor in skin angiogenesis. J Dermatol Sci. 41(1): 11-9.
  • Size / Price
    货号: MG50125-NH
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    库存2-3 weeks
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