|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), C端GFPSpark标签||MG51754-ACG|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), C端OFPSpark标签||MG51754-ACR|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), N端GFPSpark标签||MG51754-ANG|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), N端OFPSpark标签||MG51754-ANR|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), C端FLAG标签||MG51754-CF|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), C端His标签||MG51754-CH|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), C端Myc标签||MG51754-CM|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), C端HA标签||MG51754-CY|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), N端FLAG标签||MG51754-NF|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), N端His标签||MG51754-NH|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), N端Myc标签||MG51754-NM|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), N端HA标签||MG51754-NY|
|Mouse PHGDH Gene cDNA clone plasmid||MG51754-U|
|小鼠 PHGDH 基因全长ORF克隆 (表达载体), 无标签||MG51754-UT|
PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.