( We provide with PRKD3 qPCR primers for gene expression analysis, MP201038 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG51080-ACG|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG51080-ACR|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG51080-ANG|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG51080-ANR|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG51080-CF|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG51080-CH|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG51080-CM|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG51080-CY|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA(克隆载体)||MG51080-G|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG51080-NF|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG51080-NH|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG51080-NM|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG51080-NY|
|小鼠 PKC-nu/PRKD3 基因ORF全长cDNA克隆(表达载体)||MG51080-UT|
Serine/threonine-protein kinase D3, also known as Protein kinase C nu type, Protein kinase EPK2, PRKD3, EPK2 and PRKCN, is a cytoplasm and membrane protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD3 / PRKCN contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PRKD3 / PRKCN converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. It is involved in resistance to oxidative stress. PRKD3 / PRKCN is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-735 and phosphorylation of Ser-731 by PKC relieves auto-inhibition by the PH domain. PRKD3 / PRKCN can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. PRKD3 / PRKCN can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phospholipase C gamma and the involvement of other PKC family members.