|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50203-ACG|
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50203-ACR|
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50203-CF|
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50203-CH|
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50203-CM|
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50203-CY|
|小鼠 PLA2G2A 基因ORF全长cDNA(克隆载体)||MG50203-G|
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50203-NF|
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50203-NH|
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50203-NM|
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50203-NY|
|小鼠 PLA2G2A 基因ORF全长cDNA克隆(表达载体)||MG50203-UT|
Phospholipase A2, membrane associated, also known as Phosphatidylcholine 2-acylhydrolase 2A, Group IIA phospholipase A2, Non-pancreatic secretory phospholipase A2 and PLA2G2A, is a peripheral membrane protein which belongs to the phospholipase A2 family. PLA2G2A is found in many cells and also extracellularly. The membrane-bound and secreted forms of PLA2G2A are identical. PLA2G2A has been proposed to play a role in anti-bacterial defense, inflammation and eicosanoid generation, in clearance of apoptotic cells, and in the Wnt signaling pathway. PLA2G2A is thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. PLA2G2A catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. PLA2G2A might be a factor in human colorectal tumorigenesis.