|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50457-ACG|
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50457-ACR|
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50457-CF|
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50457-CH|
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50457-CM|
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50457-CY|
|小鼠 Prolactin Receptor 基因ORF全长cDNA(克隆载体)||MG50457-M|
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50457-NF|
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50457-NH|
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50457-NM|
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50457-NY|
|小鼠 Prolactin Receptor 基因ORF全长cDNA克隆(表达载体)||MG50457-UT|
Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type â… cytokine receptor superfamily, and contains two fibronectin type-â…¢ domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules invloved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.