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小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-His 标签

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    小鼠 PTPN11 cDNA Clone产品信息
    NCBI注册码:NM_001109992.1
    参考序列ORF长度:1782bp
    cDNA基因描述:Full length Clone DNA of Mus musculus protein tyrosine phosphatase, non-receptor type 11, transcript variant 2 with C terminal His tag.
    分子别称:Syp, Shp2, PTP1D, PTP2C, SAP-2, SHP-2, SH-PTP2, SH-PTP3
    分子种属:Mouse
    载体:pCMV3-C-His
    质粒:
    限制性酶切位点:
    Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with PTPN11 qPCR primers for gene expression analysis, MP200465 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    His Tag Info

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

    Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-His 标签 on other vectors
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG50462-ACG2070
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG50462-ACR2070
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签MG50462-ANG2070
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签MG50462-ANR2070
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG50462-CF1890
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-His 标签MG50462-CH1890
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG50462-CM1890
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG50462-CY1890
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA(克隆载体)MG50462-M920
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG50462-NF1890
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-His 标签MG50462-NH1890
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG50462-NM1890
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG50462-NY1890
    小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体)MG50462-UT1890
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    研究背景

    SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.

    参考资料
  • Ganju R K, et al. (2000) Beta-chemokine receptor CCR5 signals through SHP1, SHP2, and Syk. J Biol Chem. 275(23):17263-8.
  • Yin T, et al. (1997) Molecular characterization of specific interactions between SHP-2 phosphatase and JAK tyrosine kinases. J Biol Chem. 272(2):1032-7.
  • Kontaridis MI, et al. (2006) PTPN11 (Shp2) mutations in LEOPARD syndrome have dominant negative, not activating, effects. J Biol Chem. 281(10):6785-92.
  • Size / Price
    货号: MG50462-CH
    目录价: 
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