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小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签

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Mouse PTPN11 cDNA Clone产品信息
NCBI注册码:NM_001109992.1
参考序列ORF长度:1782bp
cDNA基因描述:Full length Clone DNA of Mus musculus protein tyrosine phosphatase, non-receptor type 11, transcript variant 2 with N terminal Flag tag.
分子别称:Syp, Shp2, PTP1D, PTP2C, SAP-2, SHP-2, SH-PTP2, SH-PTP3
分子种属:Mouse
载体:pCMV3-N-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签 on other vectors
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG50462-ACG2070
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG50462-ACR2070
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签MG50462-ANG2070
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签MG50462-ANR2070
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG50462-CF1890
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-His 标签MG50462-CH1890
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG50462-CM1890
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG50462-CY1890
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA(克隆载体)MG50462-M920
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG50462-NF1890
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-His 标签MG50462-NH1890
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG50462-NM1890
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG50462-NY1890
小鼠 SHP2 / PTPN11 transcript variant 2 基因ORF全长cDNA克隆(表达载体)MG50462-UT1890
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研究背景

SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.

参考资料
  • Ganju R K, et al. (2000) Beta-chemokine receptor CCR5 signals through SHP1, SHP2, and Syk. J Biol Chem. 275(23):17263-8.
  • Yin T, et al. (1997) Molecular characterization of specific interactions between SHP-2 phosphatase and JAK tyrosine kinases. J Biol Chem. 272(2):1032-7.
  • Kontaridis MI, et al. (2006) PTPN11 (Shp2) mutations in LEOPARD syndrome have dominant negative, not activating, effects. J Biol Chem. 281(10):6785-92.
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    货号: MG50462-NF
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